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Microscopes

Microscopes

​Cells are VERY small. In order to see them you have to use a microscope. Watch this video to learn about how to use a microscope.
​Microscopy
Cells vary in size. With few exceptions, individual cells cannot be seen with the naked eye, so scientists use microscopes (micro- = “small”; -scope = “to look at”) to study them. A microscope is an instrument that magnifies an object. Most photographs of cells are taken with a microscope, and these images can also be called micrographs.

​Light microscopes
Most student microscopes are classified as light microscopes. Visible light passes and is bent through the lens system to enable the user to see the specimen. Light microscopes are advantageous for viewing living organisms, but since individual cells are generally transparent, their components are not distinguishable unless they are colored with special stains. Staining, however, usually kills the cells.

Electron microscopes
In contrast to light microscopes, electron microscopes use a beam of electrons instead of a beam of light. Not only does this allow for higher magnification and, thus, more detail , it also provides higher resolving power. The method used to prepare the specimen for viewing with an electron microscope kills the specimen.  Living cells cannot be viewed with an electron microscope. As you might imagine, electron microscopes are significantly more bulky and expensive than light microscopes.
Picture
Figure 1 . (a) Most light microscopes used in a college biology lab can magnify cells up to approximately 400 times and have a resolution of about200 nanometers. (b) Electron microscopes provide a much higher magnification, 100, 000x, and a have resolution of 50 picometers. (credit a:modification of work by "GcG"/wikimedia commons; credit b: modification of work by evan )
​Cell size
At 0.1–5.0 µm in diameter, prokaryotic cells are significantly smaller than eukaryotic cells, which have diameters ranging from 10–100 µm. The small size of prokaryotes allows ions and organic molecules that enter them to quickly spread to other parts of the cell. Similarly, any wastes produced within a prokaryotic cell can quickly move out. However, larger eukaryotic cells have evolved different structural adaptations to enhance cellular transport. Indeed, the large size of these cells would not be possible without these adaptations. In general, cell size is limited because volume increases much more quickly than does cell surface area. As a cell becomes larger, it becomes more and more difficult for the cell to acquire sufficient materials to support the processes inside the cell, because the relative size of the surface area through which materials must be transported declines.
Picture
Figure 2. This figure shows relative sizes of microbes on a logarithmic scale (recall that each unit of increase in a logarithmic scale represents a 10-fold increase in the quantity being measured). Source: https://www.quora.com/Do-atoms-make-up-cells
Addapted from: ​Human Biology Copyright © by Nancy Barrickman; Kathy Bell, DVM, MPH; and Chris Cowan, M.S. is licensed under a Creative Commons Attribution 4.0 International License, except where otherwise noted.
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  • Home
  • Biology
    • Matter and Energy >
      • Honors Matter and Energy
      • Burning Ethanol Lab
    • Molecules of Life >
      • Honors Biomolecules
      • Digestion Biosynthesis
      • Microscopes
      • Cell Labs
    • Cellular Respiration >
      • Mealworm Labs
    • Cancer and the Cell Cycle >
      • Hematopoiesis
      • HLA Matching
    • The Central Dogma >
      • Double Helix
      • HONORS: DNA to Proteins
      • Yeast Sphere Lab
    • Genetics >
      • Mendelian Genetics
      • Meiosis
      • Pedigree Analysis
    • Evolution >
      • Battling Bacteria
      • Mouse Evolution
      • Speciation
      • Evidence of Evolution
    • Plants >
      • Plant Labs
    • Ecology >
      • Animal Flash Cards
      • Nutrient Cycles
      • Human Impacts on Environment
      • Carbon Pools Reading
  • SEP labs
    • Transformation >
      • Transformation Procedure
    • HLA Sequencing
    • PCR
    • ELISA
    • DNA Extraction Cards
  • Data
    • Graphing
    • Relationships in Data